Composite

Part:BBa_I716319:Design

Designed by: Hannah Cole   Group: iGEM07_Berkeley_UC   (2007-07-24)


Myoglobin gene attached to 1092 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 331
    Illegal XbaI site found at 367
    Illegal XbaI site found at 420
    Illegal PstI site found at 640
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 331
    Illegal PstI site found at 640
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 625
    Illegal EcoRI site found at 331
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 331
    Illegal XbaI site found at 367
    Illegal XbaI site found at 420
    Illegal PstI site found at 640
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 331
    Illegal XbaI site found at 367
    Illegal XbaI site found at 420
    Illegal PstI site found at 640
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1727


Design Notes

Coudln't use EcoRI because it is within the myoglobin gene.


Source

I716318 and pBca9145-Bca1092

References